Fig. 2: Morphology of the MCF10A p53 mutant-induced mammospheres. | npj Breast Cancer

Fig. 2: Morphology of the MCF10A p53 mutant-induced mammospheres.

From: Multidimensional quantitative phenotypic and molecular analysis reveals neomorphic behaviors of p53 missense mutants

Fig. 2

a Low-magnification image (scale bar: 100 μm) of the mammospheres formed by p53 missense mutants in Matrigel by on-top method stained for nucleus (DAPI, blue), laminin (green), and β-catenin (red). The cell lines were ordered by the degree of polarization shown below in (b) which shows the hollowness and laminin staining of the mammospheres. The nuclear DAPI intensity was measured in 20 concentric bins from the center of the mammospheres (left panel). The right panel shows the laminin staining intensity profiles across the bins, where laminin signal intensity in the inner bins indicate disruption of cell polarity. The intensity value data was normalized to the total number of pixels for each spheroid.

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