Fig. 5: The Hippo/YAP/TAZ pathway is strongly associated with invasiveness of cells expressing different p53 mutants.

a Biplots for a PLSR model trained on ssGSEA scores of the entire pathway terms in WikiPathways database and the vector of invasiveness is shown. The model was based on top 5 pathway terms that were identified by cross-validated forward feature selection. Cell lines (colored by invasiveness) were transformed and projected on a 2-component space, and the explained variance of invasiveness by each component is shown in the axis labels. The loadings of pathway terms were scaled to fit the data range and displayed as green lines. b Biplot for a PLSR model trained on expression values of genes in the Hippo-YAP Signaling Pathway (WikiPathways) and the vector of invasiveness is shown. Analysis performed as described for Fig. 5a. c In the WikiPathways diagrams for the Hippo/YAP/TAZ pathways, individual genes were color-coded by the Pearson correlations between gene expression levels and invasiveness across the 13 cell lines. d Transcriptional activity of TEAD proteins was measured in the 13-cell line panel in triplicates by a cell-based luciferase reporter assay. The luminescence values were normalized to the log2 fold changes over the WT^OE values. The difference between a group of two more invasive cells (R273C and Y220C) and four less invasive cells (Y234C, WT, H179R, and G245S) was tested by the two-sided Student’s t-test. Pearson’s correlation between the normalized TEAD activities and the invasiveness across the cell lines was also calculated (bottom box).