Fig. 2: MUC1-C regulates ER coactivator SRC-3 by an MK2-mediated mechanism.

a Nuclear lysates from MCF-7 cells treated with 100 nM 17β-estradiol (E2) for 3 h were precipitated with anti-MUC1-C or a control IgG. The precipitates and input lysate were immunoblotted with antibodies against the indicated proteins. b Nuclear lysates from MCF-7 cells treated with 100 nM E2 for 3 h were precipitated with anti-SRC-3 or a control IgG. The precipitates and input lysate were immunoblotted with antibodies against the indicated proteins. c, d Lysates of MCF-7/tet-MUC1shRNA and T47D/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. e MCF-7/CshRNA and MCF-7/SRC-3shRNA#1 cells were analyzed for the indicated transcripts by qRT-PCR. The results (mean±SD of 4 determinations) are expressed as relative levels compared to that obtained for CshRNA cells (assigned a value of 1). f MCF-7 and T47D cells treated with vehicle or 5 μM GO-203 were analyzed for colony formation. Shown are representative photomicrographs of stained colonies (left). The results (mean±SD of three determinations) are expressed as relative colony formation compared to that for vector cells (assigned a value of 1)(right). g MCF-7/CshRNA, MCF-7/SRC-3shRNA#1 and MCF-7/SRC-3shRNA#2 cells were analyzed for colony formation. Shown are representative photomicrographs of stained colonies (left). The results (mean±SD of three determinations) are expressed as relative colony formation compared to that for vector cells (assigned a value of 1)(right).