Fig. 4: ER(Y537S) mutant cells are MUC1-C dependent. | npj Breast Cancer

Fig. 4: ER(Y537S) mutant cells are MUC1-C dependent.

From: MUC1-C dependency in drug resistant HR+/HER2− breast cancer identifies a new target for antibody-drug conjugate treatment

Fig. 4: ER(Y537S) mutant cells are MUC1-C dependent.

a Nuclear lysates from MCF-7(Y537S) cells grown in hormone deprived conditions for 2 days were precipitated with anti-MUC1-C or a control IgG. The precipitates and input lysate were immunoblotted with antibodies against the indicated proteins. b Lysates from MCF-7(Y537S)/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. c Lysates from MCF-7(Y537S) cells treated with vehicle or 5 μM GO-203 for 2 days were immunoblotted with antibodies against the indicated proteins. d, e Chromatin from MCF-7/tet-MUC1shRNA (d) and MCF-7/ER(Y537)/tet-MUC1shRNA (e) cells treated with vehicle or DOX for 11 days were immunoblotted with antibodies against the indicated proteins. f MCF-7(Y537S)/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for MUC1-C, TFF1, MYB and BCL2 transcripts by qRT-PCR. The results (mean ± SD of four determinations) are expressed as relative levels compared to that obtained for vehicle-treated cells (assigned a value of 1). g MCF-7/ER(Y537S)/tet-MUC1shRNA cells treated with vehicle or DOX were analyzed for colony formation. Shown are representative photomicrographs of stained colonies (left). The results (mean±SD of three determinations) are expressed as relative colony formation compared to that for vehicle-treated cells (assigned a value of 1) (right). h MCF-7(Y537S) cells treated with vehicle or 5 μM GO-203 were analyzed for colony formation. Shown are representative photomicrographs of stained colonies (left). The results (mean ± SD of three determinations) are expressed as relative colony formation compared to that for vehicle-treated cells (assigned a value of 1)(right).

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