Fig. 5: Mutual regulation of the m6A methylation complex components.
From: Functional and clinical significance of the RNA m6A methyltransferase complex in breast cancer
Effect of siRNA-mediated knockdown of METTL3, METTL14 and CBLL1, both individually and in combination, on expression of METTL3, METTL14 and CBLL1 in MDA-MB-231. Quantification of expression of CBLL1, METTL3 and METTL14 determined by western blot with β-Actin loading control (n = 6) (A-I). Protein expression quantification of: (A) CBLL1 following siRNA-mediated knockdown of CBLL1; (B) METTL3 following CBLL1 siRNA-mediated knockdown; (C) METTL14 following CBLL1 siRNA-mediated knockdown. D CBLL1, (E) METTL3 and (F) METTL14 expression following siRNA-mediated knockdown of METTL3 and knockdown of METTL3 and CBLL1 in combination. G CBLL1, (H) METTL3 and (I) METTL14 expression following siRNA-mediated knockdown of METTL14 and knockdown of METTL14 and CBLL1 in combination. Evidence of the regulation of (J) METTL3, (K) CBLL1 and (L) METTL14 by m6A methylation in MDA-MB-231, MeRIP-seq data obtained from GEO series GSE185494. Peaks in second panel (m6A enriched) compared to first panel (no enrichment control) show area of m6A enrichment within gene. Red box highlights last exon of each gene and yellow box highlights the third exon of METTL3. * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001, **** = p ≤ 0.0001 by t-test or ANOVA for multiple comparisons bar charts show mean ± SEM.
