Fig. 1

PcTF expression stimulates upregulation of known targets of Polycomb in transiently transfected cells. a Map of the PcTF-expressing plasmid (top). The natural PRC1 complex mediates gene silencing (middle). PcTF expression leads to accumulation of PcTF at H3K27me3 and gene activation (bottom).¹¹ b Transiently-transfected U-2 OS, SK-N-SH, and K562 cells were visualized via the mCherry red fluorescent protein (RFP) tag. qRT-PCR was used to determine mRNA levels of fusion protein transcripts (PcTF or ΔTF) and a panel of 14 target genes at 24, 48, and 72 h post-transfection. RFP signal was not detected in K562 after 48 h, therefore later time points were omitted for K562 in this assay and other experiments. The heat map shows scaled, average log₂ fold change ratios for GAPDH-normalized expression in plasmid-transfected cells compared to cells mock-transfected with the vehicle (Lipofectamine LTX) only. Standard deviations are shown in Fig. S1