Fig. 3
From: Regulation of cancer epigenomes with a histone-binding synthetic transcription factor
Polycomb-repressed genes become activated by PcTF in a dose-dependent manner. a The illustration shows doxycycline (dox) induced PcTF expression in U2OS-PcTF cells, leading to accumulation of PcTF at H3K27me3-positive promoters and gene activation.11 b Flow cytometry analysis of dox-treated cells. Grey bars show average RFP signal (n = 3, Error = standard deviation). RFP signal histograms and RFP-positive cell frequency are shown in Fig. S4. c qRT-PCR analysis of PcTF and eight genes that showed PcTF-specific activation in transiently transfected U-2 OS cells. The heat map shows average (n = 3) log2 fold change ratios for GAPDH-normalized expression in dox-treated cells compared to one of the untreated replicates. R2 = Pearson correlation coefficient of average log2 fold change values for target genes vs. PcTF
