Fig. 4
From: Regulation of cancer epigenomes with a histone-binding synthetic transcription factor
ChIP-PCR and ChIP-seq analysis of PcTF and H3K27me3 distribution in U2OS-PcTF cells. a The map shows the location of primer pairs and amplicons (Table S3) used to analyze DNA from IP-enriched chromatin. Heat maps show averages of triplicate qPCR reactions from duplicate (PolII, H3K4me3) or triplicate (Myc, H3K27me3) immunoprecipitations. Standard deviations are shown in the bar graphs in Fig. S6. b The Venn diagram compares genes that have PcTF- and H3K27me3-marked TSS regions (−5 to +5 kb). TSS-centered plots show the total ChIP hotspot signal value within a sliding window of 200 bp (step size = 50 bp)
