Fig. 3

Mutational profile of variants detected by targeted sequencing. VAFs of candidate mutations detected by WGS were assessed at 10 time points starting 7 days before treatment (of these, two time points were obtained before treatment and the remaining eight during therapy). This data was analysed in tandem with CT scan imaging of the patient taken over the same time period. Estimated volumes from CT imaging are represented as circles scaled by relative proportion in size. Two different major subclones defined by hierarchical agglomerative clustering are highlighted in blue and red