Fig. 2: AKT2 is a target of miR-194.

A Venn diagram (http://bioinformatics.psb.ugent.be/webtools/Venn/) of databases of microT (http://diana.imis.athena-innovation.gr/DianaTools/index.php?r=microT_CDS/), mirDIP (http://ophid.utoronto.ca/mirDIP/), and TargetScan (http://www.targetscan.org/vert_72/) to predict target genes of miR-194; B correlation and interaction analysis of target genes through STRING website (https://string-db.org/). The abscissa indicates the number of interacting genes, and the ordinate indicates the names of candidate genes; C AKT2 expression in GC and normal samples in the TCGA database. The abscissa represents the sample type, T represents the GC sample, N represents the normal sample, and the ordinate represents the sample expression level; D qRT-PCR to determine the expression of AKT2 mRNA in GC tissues and adjacent normal tissues; E Correlation analysis of miR-194 expression and AKT2 level; F Prediction of the binding site between AKT2 and miR-194 through the RNA22 website (https://cm.jefferson.edu/rna22/); G Dual luciferase assay to verify the targeting relationship between AKT2 and miR-194; H qRT-PCR to measure the silencing efficiency of AKT2; I qRT-PCR to determine miR-194 and AKT2 expression in each group of transfected cells; J western blot assay to determine the protein expression of AKT2 in transfected cells of each group. *p < 0.05, **p < 0.00, ***p < 0.0001. The measurement data are summarized by mean ± standard deviation. Data in C, D were analyzed by paired t test. Data in H–J were analyzed by one-way ANOVA with Tukey post hoc test. Data in G were analyzed by two-way ANOVA with Tukey post hoc test. Pearson coefficient was employed to evaluate the correlation between AKT2 and miR-194. Cellular experiment was repeated three times.