Fig. 4: DANCR acts as a ceRNA to sponge miR-194, thereby downregulating its expression in GC. | npj Genomic Medicine

Fig. 4: DANCR acts as a ceRNA to sponge miR-194, thereby downregulating its expression in GC.

From: KLF5 activates lncRNA DANCR and inhibits cancer cell autophagy accelerating gastric cancer progression

Fig. 4

A qRT-PCR to measure the expression of miR-194 in GC tissue and adjacent normal tissues (n = 86); B correlation analysis between miR-194 expression and DANCR level; C the binding site between DANCR and miR-194 predicted through the Blast website (https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=MegaBlast&PROGRAM=blastn&BLAST_PROGRAMS=megaBlast&PAGE_TYPE=BlastSearch&BLAST_SPEC=blast2seq&DATABASE=n/a); D dual luciferase report assay to verify the targeting relationship between DANCR and miR-194; E RNA pull-down assay to verify that DANCR specifically sponged miR-194; F qRT-PCR to measure the expression of DANCR and miR-194 in transfected cells. p < 0.05, ***p < 0.0001. The measurement data are summarized by mean ± standard deviation. Data in A were analyzed by paired t test, while those in F were analyzed by unpaired t test. Data in E were analyzed by one-way ANOVA with Tukey post hoc test. Pearson coefficient was employed to evaluate the correlation between miR-194 and lncRNA DANCR. Cellular experiment was repeated three times.

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