Fig. 5: DANCR knockdown upregulates miR-194 expression, thereby accelerating GC cell autophagy. | npj Genomic Medicine

Fig. 5: DANCR knockdown upregulates miR-194 expression, thereby accelerating GC cell autophagy.

From: KLF5 activates lncRNA DANCR and inhibits cancer cell autophagy accelerating gastric cancer progression

Fig. 5

A qRT-PCR to measure the mRNA expression of DANCR and miR-194 in transfected cells; B MTT assay to detect the viability of transfected cells; C Quantitative analysis of autophagy of transfected cells under the observation of transmission electron microscope; D western blot assay to measure the expression of autophagy-related proteins (LC3-I, LC3-II, and Beclin-1); E TUNEL assay to assess TUNEL-positive apoptotic cells; F Flow cytometric detection of apoptosis rate of the transfected cells. *p < 0.05, **p < 0.00, ***p < 0.0001. The measurement data are summarized by mean ± standard deviation. Data in A–F were analyzed by one-way ANOVA with Tukey post hoc test. Statistical analysis in relation to time-based measurements in B was realized using repeated measures ANOVA, followed by a Bonferroni’s post hoc test. Cellular experiment was repeated three times.

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