Fig. 6: KLF5 knockdown promotes GC cell autophagy by regulating the DANCR/miR-194/AKT2 axis. | npj Genomic Medicine

Fig. 6: KLF5 knockdown promotes GC cell autophagy by regulating the DANCR/miR-194/AKT2 axis.

From: KLF5 activates lncRNA DANCR and inhibits cancer cell autophagy accelerating gastric cancer progression

Fig. 6

A qRT-PCR to measure the expression of KLF5, DANCR, miR-194, and AKT2 in transfected cells; B MTT assay to detect the viability of transfected cells; C quantitative analysis of autophagy of transfected cells under the observation of transmission electron microscope; D western blot assay to measure the expression of KLF5, AKT2, and autophagy-related proteins (LC3-I, LC3-II, and Beclin-1) in transfected cells; E TUNEL assay to detect TUNEL-positive apoptotic cells; F flow cytometry to evaluate the apoptosis rate of transfected cells. *p < 0.05, **p < 0.00, ***p < 0.0001. The measurement data are summarized by mean ± standard deviation. Data in A, C–F were analyzed by one-way ANOVA with Tukey post hoc test. Statistical analysis in relation to time-based measurements in B was realized using repeated measures ANOVA, followed by a Bonferroni’s post hoc test. Cellular experiment was repeated three times.

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