Fig. 5: RNA-seq analysis reveals genes and pathways targeted by TBX20. | npj Genomic Medicine

Fig. 5: RNA-seq analysis reveals genes and pathways targeted by TBX20.

From: Loss of grand histone H3 lysine 27 trimethylation domains mediated transcriptional activation in esophageal squamous cell carcinoma

Fig. 5: RNA-seq analysis reveals genes and pathways targeted by TBX20.The alternative text for this image may have been generated using AI.

a Kaplan–Meier estimates of overall survival of patients with low- or high-expression level of TBX20 in TCGA ESCC patients (left) and with mutated or not mutated TBX20 in TCGA Pan-Cancer (right). Statistical analysis was performed using the log-rank test and univariate Cox analysis. b Genomic snapshot of H3K27me3, H3K4me3, H3K4me1, H3K27ac, and RNA-seq in NE2, KYSE450, and KYSE510 at the TBX20 loci. c Experimental design to test the role of GSDs mediated oncogenesis candidates in cell proliferation and subcutaneous tumor growth. d Cell proliferation was measured using the CCK-8 assay. Error bars, s.d. calculated from five replicates. ***P < 0.001, **P < 0.01, **P < 0.05, compared to control siRNA in Wilcoxon rank sum test. e TBX20 silencing inhibited tumor growth as measured by tumor weight. Error bars, s.d. calculated from night replicates. ***P < 0.001, compared to control in Wilcoxon rank sum test. f MA plot comparing control and TBX20 knockdown KYSE510 cells. g Heat map of Metascape-enriched clusters. Each cluster contains multiple gene sets to eliminate redundancy. Analysis used genes meeting an FDR q < 0.05 threshold.

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