Fig. 2: Validation for spike-in molecules with 5′ protruding jagged ends. | npj Genomic Medicine

Fig. 2: Validation for spike-in molecules with 5′ protruding jagged ends.

From: High-resolution analysis for urinary DNA jagged ends

Fig. 2: Validation for spike-in molecules with 5′ protruding jagged ends.The alternative text for this image may have been generated using AI.

a–d Sequencing base compositions for spike-in sequence with known jagged ends. The partial sequence of the spiked-in sequences with a 1-nt and a 14-nt jagged end are indicated on the x-axis. The nucleotides denoted in the uppercase letters indicate that the sequences are in double-stranded form. The nucleotides in lowercase letters indicate that the sequences are newly filled during the end-repair process. Vertical bars with blue color and red color represent the frequencies of sequenced T and C, respectively. Sequencing results from the refined (a, c) and original version (b, d) of Jag-seq are shown.

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