Fig. 1: Genome-wide DNA methylation analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences.

a Schematic overview showing sample collection from IVF neonates from the G5 versus HTF RCT as well as the inclusion of naturally conceived neonates, genome-wide DNA methylation data generation, data processing and analyses included in this study. b PCA of all CpG sites passing our QC criteria in data from UCB samples of IVF neonates that underwent embryo culture in G5 (gold) or HTF (blue) medium. c Density plot showing the distribution of beta values from all sites and samples within each group (G5 = gold, HTF = blue).