Fig. 4: Ano5 positively regulates osteoclast differentiation.

a The proliferation of WT, Ano5^−/− BMMs, and those transduced with a control vector or Ano5 overexpression lentivirus by CCK-8 assay. b Representative images and quantification of TRAP staining after 5–7 days of osteoclast differentiation. Scale bar, 50 µm. c Relative mRNA expression of Trap, Nfatc1, Cfos, and Ctsk in osteoclasts differentiated from WT, Ano5^−/− BMMs and d those transduced with a control vector or Ano5 overexpression lentivirus. All results are representative of data generated from at least three independent experiments. Data are represented as the mean ± SEM. Two-tailed Student’s t test (for c) and two-way ANOVA followed by Tukey’s multiple comparisons test (for a, b, d). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ns: not significant P > 0.05.