Fig. 4: Startling reaction to trains of vibrations, increased seizure prevalence and increased post-seizure recovery time in flies with Oatp30B knockdown.

a Startling reaction to trains of vibrations. The UAS-Oatp30B^IR (GD12775) transgenic or the control UAS-GFP^IR were driven with nSyb-Gal4 and Ubi-Gal80ts. The w¹¹¹⁸ strain is a control for the genetic background in absence of transgenes. Mean ±SEM **P < 0.01, One Way ANOVA, Tukey’s post-hoc test. Units are the vibration events experienced 6 times for each fly, n = 174–210. b Increased seizure prevalence. The UAS-Oatp30B^IR (GD12775) transgenic or the control UAS-GFP^IR were driven with nSyb-Gal4 and Ubi-Gal80ts. Percent ±SE ****P < 0.0001, Log-rank (Mantel-Cox) test, X² 24.68 for 1 df, n = 34–36. c Increased post-seizure recovery time. The UAS-Oatp30B^IR (GD12775) transgenic or the control UAS-GFP^IR were driven with nSyb-Gal4 and Ubi-Gal80ts. Mean ±SEM *P < 0.05, Mann Whitney non-parametric test, two tails, n = 10–26. Only flies that displayed a seizure within 120 s as in b have been included in the analysis.