Fig. 2: Loss of Drosophila photoreceptor EGFP expression patterning in sln RNAi-knockdown eyes by Day 14.

Rhodopsin 1 EGFP labeling of photoreceptor rhabdomeres visualized by fluorescent deep pseudopupil (DPP) and optic neutralization imaging of the cornea (ONC) at days 1, 7, and 14 for negative controls (A–C) Low magnification DPP (4×) and (D) high magnification ONC (40×) imaging of RNAi knockdowns for mCherry (RNAi-BL35785). E–G RNAi knockdown for sln (VDRC#: 109464). D, H Only a subset of EGFP + PR are in focus under the microscope due to the curvature of the compound eye. Bottom panel graphs showing percentage of flies with intact fluorescent DPP. In all negative controls tested (left graph), 70–100% of flies had intact DPP by day 14 depending on the RNAi line and therefore a 70% threshold was set for phenotypes. Two separate knockdown experiments using silnoon (sln) RNAi-VDRC#109464 lines revealed 10% and 0% of flies had intact DPP by day 14 (Bottom: middle and right graphs). For each RNAi line tested n = 10 for each days 1, 7, and 14 timepoints. Scale bars: A–C, E–G = 50 µm; D and H = 10 µm.