Fig. 4: Morphology evaluation. | npj Microgravity

Fig. 4: Morphology evaluation.

From: Testing 3D printed biological platform for advancing simulated microgravity and space mechanobiology research

Fig. 4

Fluorescence images of GBM and HUVEC cells, showing actin filaments (red), VE-Cadherin (green) and DAPI (blue) after 24 h of gravity (1 G) (A, E) and μG (C, G) conditions respectively. Box plots showing the changes of surface area (B, F) and histograms presenting Shape Index (SI) together with Tortuosity Index (TI) (D, H) for A-172 and HUVEC cells respectively, at different experimental condition. Scale bars, 100 µm. Data are shown as the mean ± SEM (n = 50). Asterisks are reported for cases with statistical significance: * GBM SI: 24 h 1 G Vs 24 h μG, p = 0.04, *** HUVEC SI an TI: 24 h 1 G Vs 24 h μG, p = 0.001. The statistical analysis of the data was performed using one-way analysis of variance (ANOVA) with Turkey’s test for multiple comparisons.

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