Fig. 5: Protein expression analysis. | npj Microgravity

Fig. 5: Protein expression analysis.

From: Testing 3D printed biological platform for advancing simulated microgravity and space mechanobiology research

Fig. 5

A Graphical rendering of the MOC showing the 3D lateral and Top view. B Example of IWB plate images showing Cell Tag 700 stain, in red, for cell number normalization and IRDye secondary antibodies, in green, for protein fluorescence signal detection. A dashed yellow circle highlights the Region of Interest (ROI) selected for analysis. IWB assay for changes in Active Yap-1 (C) and Vinculin (D) proteins expression in response to 24 h of μG condition, in Glioblastoma A-172. IWB assay for changes in Vinculin (E) and VE-Cadherin (F) proteins expression in response to 24 h of μG condition, in HUVECs. Data are shown as the mean ± SEM (n = 3). Asterisks are reported for cases with statistical significance: * YAP-1 24 h 1 G Vs 24 h μG: p = 0.329, ** VE-Cadherin 24 h 1 G Vs 24 h μG: p = 0.013. The statistical analysis of the data was performed using one-way analysis of variance (ANOVA) with Turkey’s test for multiple comparisons.

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