Fig. 6: Junction development evaluation.

Fluorescence images of a sub-confluent monolayer of HUVECs showing Actin-Filament. (red), VE-Cadherin (green) and Dapi (blue) at Time 0 (A) and after 24 h of gravity (1 G) (B) and μG (C) conditions. D Fluorescence. profile of VE-Cad patter along the perimeter of the cell for 1 G and μG sample. The positive intensity represents junctional protein formation while the negative intensity indicates gaps between cells. E Histogram showing the percentage of positively stained VE-Cad along the entire cell border for all experimental conditions. Scale bars, 50 µm. Data are shown as the mean ± SEM (n = 50). Asterisks are reported for cases with statistical significance: **** 24 h 1 G Vs 24 h μG, p = 0.0001. The statistical analysis of the data was performed using one-way analysis of variance (ANOVA) with Turkey’s test for multiple comparisons.