Fig. 5: Schematic diagram of protocol of loading worms into NemaFlex-S device using the Worm Loading Apparatus. | npj Microgravity

Fig. 5: Schematic diagram of protocol of loading worms into NemaFlex-S device using the Worm Loading Apparatus.

From: Microfluidics-integrated spaceflight hardware for measuring muscle strength of Caenorhabditis elegans on the International Space Station

Fig. 5

a Shows the schematic of the worm loading apparatus. (b) Step I: priming of the valve with CeMM, flow will be from CeMM media syringe to waste collection syringe. (c) Step II: aspirating a specified worm aliquot from worm culture bag to worm suspension loop. d Step III: priming of the valve with worm suspension, flow is from suspension loop to waste collection syringe. (e) Step IV: taking a specified worm aliquot (400 µL) from worm bag to suspension loop. (f) Step V: load the worms into A side of the NemaFlex-S device. Repeat step IV and step V to load the worms into the B side of the NemaFlex-S device.

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