Fig. 3: Effect of unloading on MyHCIIx myosin expression in Vastus Lateralis and Gastrocnemius.
From: Time-dependent unloading effects on muscle and bone and involvement of FNDC5/irisin axis
Quantitative PCR (qPCR) showing mRNA expression levels of MyHCIIx in Vastus Lateralis (a–d), and Gastrocnemius (e–h) muscles of control mice kept in resting condition (Rest) and HU mice (Unload) at time points 1-2-3 and 4 weeks. Analysis showed a down-regulation of MyHCIIx at 1 week of suspension in both Vastus Lateralis (a) and Gastrocnemius (e) muscles. The decrease of MyHCIIx was also observed at 3 and 4 weeks of suspension in Vastus Lateralis (c) and Gastrocnemius (h), respectively. Shapiro-Wilk test and Mann-Whitney test were performed. Data are presented as box-and-whisker with median and interquartile ranges, from max to min, with all data points shown. *p < 0.05, ***p < 0.001, ****p < 0.0001 vs Rest.
