Fig. 4: Immunofluorescence staining of phalloidin for actin structures (orange), vinculin for focal adhesions (green), and DAPI for cell nucleus (blue).
From: Gravitational effects on fibroblasts’ function in relation to wound healing
a 1 × g control fibroblasts. b Fibroblasts exposed to 15 × g hypergravity. c Fibroblasts exposed to 20 × g hypergravity. d Fibroblasts exposed to simulated microgravity. e Fibroblasts exposed to 15 × g hypergravity transitions. f Fibroblasts exposed to 20 × g hypergravity transitions. Solid white arrows show loss of perinuclear actin fibers in fibroblasts exposed to simulated microgravity (either with or without hypergravity exposure). Open arrow shows bending of perinuclear actin fibers surrounding the nucleus. Scale bar = 50 µm. smg = simulated microgravity, 15 × g T and 20 × g T are hypergravity transition groups.
