Fig. 2: Hindlimb suspension affects gastrocnemius muscles.

a Assessment of mouse body weights at the end of the experimental study show no gross difference between groups. b Images of dissected hindlimb muscles (tibialis anterior, gastrocnemius and quadriceps) from all groups. c–e Ratio of gross muscle weights, divided by the total body weight of the respective mouse. c tibialis anterior, d quadriceps, e gastrocnemius. f Representative images of gastrocnemius muscle fibers stained with fluorescently labeled WGA, which marks muscle fiber boundaries. Scale bar: 100 μm. g Enumeration of muscle fiber cross-sectional areas (CSA) from gastrocnemius muscles. At least 4 mice per condition were analyzed. Represented are mean ± SEM for column graphs and median with 25th and 75th quartiles marked for violin plots. Data were analyzed using one-way ANOVA and Tukey’s multiple comparison test of means. Adjusted p values are displayed.