Fig. 3: Telomere length assessments in gastrocnemius myogenic cells. | npj Microgravity

Fig. 3: Telomere length assessments in gastrocnemius myogenic cells.

From: Impacts of radiation exposure, hindlimb unloading, and recovery on murine skeletal muscle cell telomere length

Fig. 3

a Representative images of MuSCs (VCAM + , in the satellite cell position; green), telomere staining (PNA probe; red) and nuclear staining (DAPI; blue). Myonuclei are nuclei residing within the myofibers and do not stain for VCAM. Scale bar: 10 μm. b Quantification of telomere length in MuSC populations of gastrocnemius muscles from control, HLS, IR, and HLS + IR mice. Telomere length was calculated as the total telomere signal intensities divided by the intensity of DAPI, to account for cell cycle positioning. c Myonuclei telomere length evaluation in gastrocnemius muscles. Three mice per experimental group were analyzed. Violin plots in this figure are depicting median with 25th and 75th quartiles. Data were analyzed using one-way ANOVA and Tukey’s multiple comparison test of means. Adjusted p values are displayed.

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