Fig. 6: Interrogation of telomere lengthening mechanisms in hindlimb unloading and recovery. | npj Microgravity

Fig. 6: Interrogation of telomere lengthening mechanisms in hindlimb unloading and recovery.

From: Impacts of radiation exposure, hindlimb unloading, and recovery on murine skeletal muscle cell telomere length

Fig. 6

a TRAP assay to assess telomerase activity in control, HLS + IR, and recovery skeletal muscle lysates. RNase treatment served as a negative control and induced pluripotent stem cell (iPSC) lysate served as a positive control. Each lane is from one gastrocnemius muscle lysate per mouse, with three biological replicates per group. b Quantification of telomere elongation via telomerase from a. Displayed are mean ± SEM. c, d Quantitative real-time PCR of ALT pathway regulators Atrx and Daxx from gastrocnemius muscles of control, HLS + IR, and recovery groups. Gapdh served as a housekeeping gene for normalization. At least three mice were examined per group. Displayed is mean ± SEM. Data were analyzed using one-way ANOVA and Tukey’s multiple comparison test of means. Adjusted p values are displayed.

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