Fig. 6: Confirmation of SMG condition generated using CS clinostat.
a Approximately 5 × 105 of the cells are seeded in the PDMS clinostat dish and incubated in 1 g for 24 h. These cells are then moved into the CS clinostat and incubated for 12, 24, and 36 h. Cells cultured in 1 g are used as controls. Microscopic images show the cells on the PDMS clinostat dish after 24 h of rotation (Magnification: 100×, scale bar: 200 μm). b Cell viability is measured using an MTT assay. The arbitrary ratio is calculated as the ratio of the OD540 value of the SMG group relative to that of the control group (1 g at 0 h) at each time point (**p < 0.01). c Total protein is extracted, and CAV-1 and SMG marker expression is visualized by means of western blot analysis with β-actin as a loading control. CAV-1 is significantly lowered in the cells cultured in the CS clinostat compared to that of the cells cultured in 1 g, indicating successful generation of SMG. d The intensities of the CAV-1 bands are normalized against that of β-actin for comparison (#p < 0.05). The data were analyzed by using the ANOVA with Turkey’s test for multiple comparisons. All error bars represent standard deviation.
