Fig. 4: P. aeruginosa biofilm thickness on SS and LIS in 1 g or microgravity.

Biofilm thickness plotted as a function of time (1-, 2-, and 3-day-old biofilms) and gravitational condition. Statistical significance specified with horizontal brackets, for comparisons between microgravity and 1 g (dashed brackets), for comparisons of SS vs LIS in microgravity (gray brackets), and for comparisons of LIS between time points (blue brackets). SS = stainless steel. LIS = lubricant impregnated material. For SS, n = 8 biological replicates each imaged in 4 fields of view. For LIS, n = 4 biological replicates each imaged in 4 fields of view. Dunn’s test with Bonferroni correction ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05. Box central line = median, bounds of box = first and third quartile, whiskers = minimum and maximum values (without outliers).