Fig. 2: Main cell functions and pathways with the highest correlation to genes that are differentially expressed in µg as compared to 1 g flight control.

Canonical pathways identified by IPA were grouped regarding their cell function: a cell proliferation, b DNA repair and c Inflammation and pathological conditions. The different individual pathways were classified according to their –log(B–H p value), a corrected p value based on the Benjamini–Hochberg method, which is a powerful tool to decrease the false discovery rate (yellow line and symbols). For each individual pathway, the percentage of genes that are downregulated, upregulated and not significantly regulated in regard to our analysis cut-offs are illustrated by color code (green, red or white, respectively). The total gene number in the pathway and the z-score calculated by IPA are also indicated, with positive and negative z-score values indicative of stimulation or inhibition, respectively. Also see Supplementary Data 4–7 describing the entire canonical pathways analysis.