Fig. 3: Comparison of the nuclear morphology and the KI67 staining levels in cells at 1 g flight control and µg. | npj Microgravity

Fig. 3: Comparison of the nuclear morphology and the KI67 staining levels in cells at 1 g flight control and µg.

From: Microgravity triggers ferroptosis and accelerates senescence in the MG-63 cell model of osteoblastic cells

Fig. 3: Comparison of the nuclear morphology and the KI67 staining levels in cells at 1 g flight control and µg.

a DAPI nucleus staining (blue) in 1 g control and µg cells. b Quantification of nuclear apparent surface in 1 g control (n = 170) and µg cells (n = 190), c showing a reduction of the size of the nuclei in µg. Fluorescence intensity variations within individual nucleus (variations between the background and bright spots inside the nucleus) was measured in 1 g control cells (n = 164) and in cells in µg (n = 146). A higher heterogeneity of DAPI staining was observed in cells in µg. d KI67 (violet) staining in 1 g control and µg cells. A strong reduction of KI67 staining was observed in µg, which is illustrated on higher magnification views of representative nuclei and confirmed by e quantification of nucleus-associated violet staining (n = 102 in 1 g, n = 135 in µg). Box plots indicate the median, with the top and bottom borders of the boxes representing the 75th and 25th percentiles, respectively, and the whiskers above and below the boxes indicating the maximal and minimal values. Statistical analyses were performed with the Mann–Whitney U test (****p value < 0.0001). Scale bars are 50 µm. a.u. arbitrary units.

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