Fig. 6: cAGO promotes cell differentiation through the Wnt/β-catenin signaling pathway.
From: TRPML1 ion channel promotes HepaRG cell differentiation under simulated microgravity conditions
A The relative nuclear import of β-catenin (red) and DAPI (blue) in HepaRG cells pre-incubated with BAPTA-AM, and YM201636, under RFC or not, as assessed by immunofluorescence staining (Con: the undifferentiated group, the other groups: differentiated with DMSO). Scale bar: 40 μm. Mean ± SD by ANOVA. B Immunofluorescence staining of AFP (red) and DAPI (blue) in HepaRG cells pre-incubated with IWR-1 under RFC conditions or not, as assessed by confocal imaging. Scale bar: 80 μm. Mean ± SD by ANOVA. The data are based on three independent experiments. *P < 0.05, **P < 0.01. C Immunofluorescence staining and quantification of AFP (red) and DAPI (blue) in HepaRG cells pre-incubated with or without SKL2001(an activator of Wnt/β-catenin signaling, MCE, HY-101085, 20 μM) under normal gravity or cAGO, as assessed by confocal imaging. Scale bar: 80 μm. Mean ± SD by ANOVA. The data are based on three independent experiments. *P < 0.05, ***P < 0.01.
