Fig. 2: SEC2 reversed microgravity enhanced bone marrow adiposity.

A Femurs isolated from Control, HLS and HLS+SEC2 were subjected IF staining using anti-perilipin antibody. Representative images of perilipin-stained femurs isolated from three groups of mice. Scale bar: 100 μM. B Femur subjected to hematoxylin & eosin staining. Representative images of hematoxylin & eosin-stained femurs harvested from Control, HLS and HLS+SEC2 groups. Scale bar: 100 μM. C, D Quantification of bone marrow adipocyte number and percentage of adipocyte volume based on hematoxylin & eosin staining (n = 6; *p < 0.05, **p < 0.01, **p < 0.001).