Fig. 1: Miniaturized multicolor fluorescence imaging system integrated with a PDMS LGP.

a Schematic diagram of each component of the proposed system. b Schematic diagram of the packaged system with green- and red-labeled cells. c The simple operating principle of the proposed system. The light transmitted from the LEDs is transmitted only to the labeled cells due to total internal reflection (TIR) on the LGP’s bottom surface, and the emission light from the labeled cells is transmitted to the CMOS image sensor. d Photographs of the packaged system, image sensor used, and example image showing the green- and red-labeled NIH 3T3 fibroblast cells using the proposed system. Scale bar: 10 mm (d-i, ii) 50 μm (d-iii).