Fig. 5: Comparison of the performance of a commercial fluorescent microscope and the miniaturized imaging system integrated with a 100-μm PDMS LGP. | npj Flexible Electronics

Fig. 5: Comparison of the performance of a commercial fluorescent microscope and the miniaturized imaging system integrated with a 100-μm PDMS LGP.

From: Miniaturized multicolor fluorescence imaging system integrated with a PDMS light-guide plate for biomedical investigation

Fig. 5: Comparison of the performance of a commercial fluorescent microscope and the miniaturized imaging system integrated with a 100-μm PDMS LGP.

a Background-subtracted images captured from the microscope according to rhodamine concentration (0.2-20 μM). b Background-subtracted images captured from the miniaturized imaging system according to rhodamine concentration (0.2-20 μM). c Comparison of the background-subtracted mean fluorescence intensity between the microscope and miniaturized imaging system according to rhodamine concentration (0.2-20 μM) (0.02 µM: ****P < 0.0001, 0.2 µM: **P = 0.007, 2 µM: ****P < 0.0001, 20 µM: ****P < 0.0001). d Comparison of the signal-to-noise ratio (SNR) between the microscope and miniaturized imaging system according to rhodamine concentration (0.2-20 μM) (0.02 µM: ***P = 0.0007, 0.2 µM: **P = 0.0016, 2 µM: ****P < 0.0001, 20 µM: ***P < 0.0002). Data are presented as mean values + /- s.d. with individual data points (white circle; n = 3 for all data, n is the number of the images). Statistical significance was tested with two-tailed unpaired t-tests. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; NS: no significant difference. Scale bar: 100 μm (a, b).

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