Fig. 5

The presence of dopaminergic TH-VUM neuron is essential for the G2019S/I2020T-mediated reduction in PER. a Proportion of flies responding when LRRK2 transgenes are expressed in different subsets of the dopaminergic neurons, using the DDC, HL9, C′ or D′ GAL4 drivers. There is no difference between the increased kinase mutants (G2019S/I2020T) and the kinase-dead construct (KD, G2019S-K1906M) with the D′ GAL4, which does not express in the TH-VUM neurons. All the other GAL4 lines tested express in the TH-VUM neurons and show a smaller response in G2019S/I2020T than in KD. Exact genotypes: + is w ^a. b Summary maps of the expression patterns of the GAL4 drivers used in a. Figures redrawn after Mao and Davis (2009).¹⁰ c The lack of TH-VUM in the D′ line is confirmed anatomically. Each panel shows the projection of a confocal stack through the sub-esophageal zone (as marked in the first panel by the dotted box). Neurons marked by expression of eIfGFP under the control of the relevant GAL4 and stained by anti-TH antibody. The SEZ contains a single anterior cell (“a”) and a group of three posterior cells (“p”), marked with anti-TH antibody With DDC, HL9, and C′ GAL4 drivers, all four SEZ neurons were GFP positive. With D′, the nucleus of the anterior neuron “a” has a weak GFP signal, but the three posterior neurons marked with anti-TH antibody do not fluoresce green (note the cytoplasm of the two left posterior cells is merged in this projection of the z-stack, but their empty nuclei are still visible). Scalebar 20 μm