Fig. 3: Bio-ELISA reliably quantifies TH in PC12 cells, human macrophages and cultured murine dopamine neurons.
From: TNFα increases tyrosine hydroxylase expression in human monocytes
a Using the Bio-ELISA shown in Fig. 1g, we quantified TH in four relevant tissues and cultured cells: PC12 (positive control), HEK293 (negative control), cultured human macrophages and cultured primary murine dopamine neurons. PC12 cells express very high levels of TH (<10 ng TH/mg total protein) relative to human macrophages (~300 pg TH/mg total protein) and primary murine dopamine neurons (~700 pg TH/mg total protein). b TH values are plotted on a representative standard curve for visual comparison, with an inset magnifying the lower end of the standard curve. c Calculations are shown by which raw TH concentration in ng/mL is normalized to total protein per sample. Samples included in a, each an independent biological replicate, are shown in c. Data are shown as ±SEM.
