Fig. 4: Absorption controls demonstrate the specificity of TH Bio-ELISA.
From: TNFα increases tyrosine hydroxylase expression in human monocytes
a Schematic layout of experimental conditions to assess absorption controls in contrast to optimized Bio-ELISA conditions using PC12 cell lysate. b Representative standard curve shown to illustrate PC12 cells’ TH concentration using optimized Bio-ELISA (blue arrow), absorbed capture antibody (MCA-4H2 preincubated with 20 µg/mL recombinant TH, orange arrow), and absorbed detection antibody (biotinylated RPCA-TH preincubated with 20 µg/mL recombinant TH, green arrow). PC12 TH is undetectable after absorption of either capture or detection antibodies, confirming assay specificity.
