Fig. 5: TH protein is increased in CD14+ monocytes isolated from PD patients.
From: TNFα increases tyrosine hydroxylase expression in human monocytes
Total CD14+ monocytes were magnetically isolated from 20 million freshly isolated PBMCs derived from whole blood of 11 PD patients and 11 healthy volunteers, immediately lysed in the presence of protease inhibitor, and stored at −80 °C. Following protein quantification, the whole lysate from each sample was added to duplicate wells and assayed for the concentration of TH. a Monocytes isolated from PD patients express a significantly greater quantity of TH compared to equivalent monocytes isolated from healthy control subjects (unpaired two-tailed T-test, alpha = 0.05, p < 0.05). b Mean TH concentration for monocytes from PD patients plotted on a representative standard curve, with inset magnifying the lower end of the curve. c Calculations are shown by which raw TH concentration in ng/mL is normalized to total protein per sample. Samples included in a, each an independent biological replicate, are shown in c. Data are shown as ±SEM.
