Table 3 Reagents and Materials.
From: TNFα increases tyrosine hydroxylase expression in human monocytes
Reagent | Supplier | Catalog number | Purpose | Concentration |
|---|---|---|---|---|
EZ-Link Sulfo-NHS-LC-Biotin | Thermo Scientific | A39257 | RPCA-TH biotinylation | 20-fold molar excess |
Fat-free milk | Carnation | N/A | WB/ELISA | 1% or 5% |
Clarity Western | BioRad | 1705061 | WB ECL | N/A |
TMB Substrate | ThermoFisher | 34028 | ELISA | Stock |
NiDAB | Vector Labs | SK-4100 | IHC | Stock |
TPA | Biolegend | 755802 | ELISA, FC | 100 ng/mL |
IL6 | Biolegend | 570802 | ELISA, FC | 17 ng/mL |
TNF-alpha | Biolegend | 570102 | ELISA, FC | 17 ng/mL |
H2SO4 | Sigma | 339741 | ELISA | 2 N |
TritonX-100 | ThermoFisher | BP151-100 | Magnetic Isolation | 1% |
Tween-20 | ThermoFisher | MP1Tween201 | TBS-T | 0.2% |
Protease Inhibitor | Millipore | 539191 | Cell lysis | 1× |
DC Protein assay | Biorad | 5000112 | Protein assay | N/A |
FBS | Gemini | 100-106 | Cell culture | 10% or 5% |
Horse serum | Sigma | H1138-500ML | Cell culture | 5% |
Pen/Strep | ThermoFisher | 15-140-122 | Cell culture | 1% |
RPMI | Corning | 10–017 | Cell culture | Stock |
Immulon 4HBX | ThermoFisher | 3855 | ELISA | N/A |
MojoSort buffer 5x | Biolegend | 480017 | Magnetic cell isolation | 1× |
Leucosep tubes | Grenier BioOne | 227290P | PBMC isolation | N/A |
Ultra-low-adherence 6-well plates | Corning | 3471 | TPA/TNF-alpha stimulation in vitro, for ELISA/FC | N/A |
Accumax | Innovative Cell Tech | AM105 | Cell detachment | Stock |
CountBright beads | Invitrogen | C36950 | FC | 5000 beads/μL |
XPro1595 | N/A | N/A | In vitro treatment | 50 ng/mL |
