Table 4 Antibodies.
From: TNFα increases tyrosine hydroxylase expression in human monocytes
Specificity | Clone/species | Conjugate | Vendor | Catalog number | Purpose | Dilution | Concentration (μg/mL |
|---|---|---|---|---|---|---|---|
TH | Polyclonal/Rabbit | N/A | Sigma | AB152 | WB | 1:1000 | 0.1 |
TH | Monoclonal/Mouse | N/A | EnCor | MCA-4H2 | WB, ELISA, IHC | 1:1000, 1:100 | 1 |
TH | Polyclonal/Rabbit | N/A | EnCor | RPCA-TH | WB, IHC | 1:1000, 1:500 | 1 |
TH | Polyclonal/Rabbit | Biotin | EnCor | RPCA-TH | ELISA | 1:6000 | 1.65 |
Chicken | Polyclonal/Rabbit | HRP | Sigma | A9046 | WB | 1:1000 | 1 |
Mouse | Polyclonal/Goat | IR-800 | Licor | 92632210 | WB | 1:15,000 | 0.0003 |
Rabbit | Polyclonal/Goat | IR-680 | Licor | 92568071 | WB | 1:15,000 | 0.0003 |
CD14 | Nanobeads | Magnetic | Biolegend | 480093 | Magnetic Isolation | 20 μL/20M cells | N/A |
Biotin | N/A (Avidin) | HRP | Vector Labs | A2004 | ELISA | 1:2500 | 0.0004 |
Mouse | Polyclonal/Goat | HRP | Biolegend | 405306 | IHC | 1:250 | 0.002 |
Isotype Ctl | IgG1,k/Mouse | N/A | Biolegend | 401401 | IHC, WB | 1:250-1000 | 1 |
Isotype Ctl | Polyclonal/Rabbit | N/A | Biolegend | 910801 | IHC, WB | 1:100–1000 | 1 |
Rabbit | Polyclonal/Donkey | HRP | Biolegend | 406401 | IHC | 1:500-1000 | 0.002 |
CD14 | Polyclonal | Magnetic | Biolegend | 480048 | ELISA, FC | Mfg Instr. | N/A |
Ki67 | Polyclonal/Chicken | N/A | Encor | CPCA-Ki67 | FC | 1:100 | 0.1 |
Chicken | Polyclonal/Goat | Alexa 488 | LifeTech | A-11039 | FC | 1:100 | 0.2 |
CD14 | IgG2b/Mouse | FITC | BD | M-Phi-09 | FC | 1:50 | 0.02 |
