Fig. 3: Pre-symptomatic Tg mice display an altered TLR-2 expression in colonic tissues and a decrease in fecal butyrate and propionate levels since 3 months of age. | npj Parkinson's Disease

Fig. 3: Pre-symptomatic Tg mice display an altered TLR-2 expression in colonic tissues and a decrease in fecal butyrate and propionate levels since 3 months of age.

From: Enteric α-synuclein impairs intestinal epithelial barrier through caspase-1-inflammasome signaling in Parkinson’s disease before brain pathology

Fig. 3

a Representative blots and (b) densitometric analysis of TLR-2 expression assessed by Western blot assay in colonic tissues from nTg and Tg mice at 3, 6, and 9 months of age. In the quantitative analysis protein levels were normalized using Ponceau staining and expressed as percentage of the mean of nTg age-matched controls. All values represent the mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001, significant difference. One-way ANOVA followed by Tukey post hoc test. c Butyrate and (d) propionate levels in the feces from nTg and Tg mice at 3, 6, and 9 months of age. All values represent the means ± SEM. Two-way ANOVA followed by Tukey post hoc test results: *P < 0.05, significant difference versus nTg age-matched controls). n = 3–5/group. nTg non-transgenic, SCFAs fermentation products of enteric bacteria, Tg transgenic, TLR-2 toll like receptor-2.

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