Fig. 5: Incubation of α-syn plus LPS directly in the medium of Caco-2 cells does not alter the expression ZO-1 and occludin. | npj Parkinson's Disease

Fig. 5: Incubation of α-syn plus LPS directly in the medium of Caco-2 cells does not alter the expression ZO-1 and occludin.

From: Enteric α-synuclein impairs intestinal epithelial barrier through caspase-1-inflammasome signaling in Parkinson’s disease before brain pathology

Fig. 5

a, b Representative blots and densitometric analysis of ZO-1 and occludin expression assessed by Western blot assay in cultured Caco-2 cells treated with conditioned medium derived from THP-1 cells treated with α-syn plus LPS, in the absence or in the presence of butyrate, or with α-syn plus LPS directly in the medium. All values represent the mean± SEM. One-way ANOVA followed by Tukey’s post hoc test results: *P < 0.05, **P < 0.01 significant difference versus Caco-2 cells (Ctrl). c Representative blots and densitometric analysis of TLR-2 expression assessed by Western blot assay in cultured THP-1 and Caco-2 cells treated with LPS and α-syn and Caco-2 incubated with conditioned medium derived from THP-1 cells treated with α-syn and LPS. All values represent the mean ± SEM. Student’s t test and one-way ANOVA followed by Tukey’s post hoc test results: no significant difference versus respective Ctrl. n = 5 independent experiments. α-syn α-synuclein, LPS lipopolysaccharide, TLR-2 toll like receptor-2, ZO-1 zonulin-1.

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