Fig. 3: BX795 reduces protein aggregates in a gene-edited p.A53T line of mature human iPSC-derived TH neurons. | npj Parkinson's Disease

Fig. 3: BX795 reduces protein aggregates in a gene-edited p.A53T line of mature human iPSC-derived TH neurons.

From: High content screening and proteomic analysis identify a kinase inhibitor that rescues pathological phenotypes in a patient-derived model of Parkinson’s disease

Fig. 3

a Representative confocal images of wild-type (ctl) and isogenic p.A53T iCell Dopa neurons immunolabelled for Nuclei, TUJ1, MAP2 and TH. Scale bar, 30 μm. b Representative confocal images of wild-type (ctl) and isogenic p.A53T iCellDopa neurons showing immunostaining for tyrosine hydroxylase (TH green) and protein aggregates (red). p.A53T cells were treated or not with BX795, as indicated. Scale bar, 5 μm. c Quantification of aggregates in TH+ neurons. Data represent mean ± SEM. (Comparisons by ANOVA with Tukey correction, ****P < 0.0001, n = at least 50 randomly selected TH+ cells for each condition).

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