Fig. 2: Assessment of week-long clenbuterol treatment on Snca transcript and α-syn protein in rats.

Rats received intraperitoneal injections of 10, 20, or 40 mg/kg clenbuterol (clen) or saline vehicle (veh) every 48 h. Tissue was collected on the 7th day, 24 h after the final injection. Prior to each injection, rats were weighed to calculate the volume of drug or vehicle required for the dose. a Snca normalized to Gapdh and measured via ddPCR. b Readily soluble α-syn fraction isolated from the SN. c Initially insoluble α-syn fraction isolated from the SN, solubilized with a stronger lysis buffer. d Readily soluble α-syn fraction isolated from the striatum. e Initially insoluble α-syn fraction isolated from the striatum, solubilized with a stronger lysis buffer. All protein fractions were measured via western blot, graphed as percent of control, and representative blots are shown below the respective graph. Columns indicate the group means, circles represent individual data points (n = 10 per group before outlier removal), error bars represent ±1 standard error of the mean. An asterisk represents significance (p ≤ 0.05). Outliers were removed based on the absolute deviation from the median method. In a, two samples were removed from the 20 mg/kg, and one sample removed from the 40 mg/kg groups. In b, two samples were removed from the 40 mg/kg group. In c and e, one sample was removed from the 40 mg/kg group. No other outliers were removed.