Fig. 1: Neuronal enrichment validation by flow cytometry and CETS analysis. | npj Parkinson's Disease

Fig. 1: Neuronal enrichment validation by flow cytometry and CETS analysis.

From: Parkinson’s disease-associated, sex-specific changes in DNA methylation at PARK7 (DJ-1), SLC17A6 (VGLUT2), PTPRN2 (IA-2β), and NR4A2 (NURR1) in cortical neurons

Fig. 1

a NeuN+ nuclei were identified using DAPI pulse area vs. width using the 405-450/45 channel for all nuclei (left) followed by NeuN-AlexaFluor488 bright events 488-525/40 (right). The percentage of NeuN+ nuclei is provided in the top-right corner. b Histogram of estimated neuronal proportion (vs. glial) for enriched PD NeuN+ nuclei population. Proportions of neuronal and glial cell types were estimated for MACS-sorted NeuN+ nuclei samples using the CETS R package during EPIC array data processing. Average proportion of neurons (vertical dotted line) was estimated to be 83.8% across all n = 99 samples included in analysis.

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