Fig. 8: Evaluation of staining pattern of aSyn pathological aggregates in WT PFF-injected mice. | npj Parkinson's Disease

Fig. 8: Evaluation of staining pattern of aSyn pathological aggregates in WT PFF-injected mice.

From: Revisiting the specificity and ability of phospho-S129 antibodies to capture alpha-synuclein biochemical and pathological diversity

Fig. 8

a Brain coronal sections (50 μM thick) from different brain areas of C57BL6/J PFF-injected mice (Amygdala, Striatum, Motor Cortex), 3 months post-injection, have been examined to assess the different staining patterns of the six pS129 antibodies (pSyn#64, MJF-R13, 81A, EP1536Y, GTX, and LASH-EGT). Scale bar = 100 μM. Quantification for each area has been carried out using Qupath and was expressed as a percentage of positive area stained of total ROI. Data are presented as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 according to one-way ANOVA followed by the Tukey test for multiple comparisons. b Microphotographs of the amygdala were stained with the six pS129 antibodies (pSyn#64, MJF-R13, 81A, EP1536Y, GTX and LASH-EGT) at higher magnification. Scale bar = 10 μM.

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