Table 4 List of antibodies used in the study, including clone information, species in which the antibody was raised and supplier.

From: Alterations in the LRRK2-Rab pathway in urinary extracellular vesicles as Parkinson’s disease and pharmacodynamic biomarkers

Detection epitope

Clone

Species

Supplier

Total LRRK2

MJFF2 (C41-2)

Rabbit

Abcam (ab133474)

Total LRRK2

UDD3 30(12)

Rabbit

Abcam (ab133518)

Total LRRK2

N241A/34

Mouse

UC Davis/NIH NeuroMab Facility (75-253)

Total LRRK2

D18E12

Rabbit

Ozyme (#13046)

Total LRRK2

MC.028.83.76.242

Mouse

BioLegend (SIG-39840-100)

pS910-LRRK2

UDD1 15(3)

Rabbit

Abcam (ab133449)

pS935-LRRK2

UDD2 10(12)

Rabbit

Abcam (ab133450)

pS955-LRRK2

MJF-R11 (75-1)

Rabbit

Abcam (ab169521)

pS973-LRRK2

MJF-R12(37-1)

Rabbit

Abcam (ab181364)

pS1292-LRRK2

MJF-R19-7-8

Rabbit

Abcam (ab203181)

Total Rab8a

D22D8

Rabbit

Ozyme (#6975)

pT72-Rab8a*

MJF-R20

Rabbit

Abcam (ab230260)

pT72-Rab8a*

Polyclonal

Rabbit

MRC PPU (E8263)

Total Rab10

D36C4

Rabbit

Ozyme (#8127)

pT73-Rab10

MJF-R21

Rabbit

Abcam (ab230261)

TSG101

4A10

Mouse

Life Technology (MA1-23296)

Alix

3A9

Mouse

Ozyme (#2171S)

CD9

EPR2949

Rabbit

Abcam (ab92726)

CD63

H-193

Rabbit

Santa Cruz (sc-15363)

GAPDH

Polyclonal

Rabbit

Sigma-Aldrich (G9545)

Beta-actin

AC-15

Mouse

Sigma-Aldrich (A5441)