Fig. 2: ATP-dependence of vesicular uptake. | npj Parkinson's Disease

Fig. 2: ATP-dependence of vesicular uptake.

From: Dopamine and vesicular monoamine transport loss supports incidental Lewy body disease as preclinical idiopathic Parkinson

Fig. 2

Dopamine uptake by synaptic vesicles prepared from striatal tissue of control (left panels) and ILBD subjects (right panels). Total uptake (closed symbols) and uptake in the presence of reserpine (open symbols) in the putamen (a, b) and in the caudate (c, d) and comparison of total uptake in control (e) and ILBD subjects (f) of the putamen (closed symbols) vs. caudate (open symbols). Synaptic vesicle preparations were incubated with 0.1 µM [3H]dopamine and MgATP at the concentrations indicated at 30 °C for 5 min in the absence or presence of 1 µM reserpine. Data are given as means ± s.e.m. (controls, n = 6; ILBD, n = 4) and additionally single values in (e, f). *p < 0.05 vs. caudate by two-way repeated measures ANOVA for the factors region (caudate or putamen) and ATP (0, 0.1, 0.4, or 2 mM).

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